ADD1 (phospho Ser726) Antibody

Product Specifications

Background

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma) . Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca (2+) /calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

NCBI Gene ID

118

Swiss Prot

P35611

Accession Number

NP_001110.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

ADD1 (Phospho-Ser726) antibody was raised against a peptide sequence around phosphorylation site of serine 726 (T-P-S (p) -F-L) derived from Human ADD1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

130 kDa

Specificity

This antibody detects endogenous level of ADD1only when phosphorylated at serine 726.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

ADD1

NCBI Official Name

Adducin 1 (alpha)

NCBI Organism

Homo sapiens

Background Reference 01

Pariser H, et al. Proc Natl Acad Sci USA 2005 Aug 30; 102 (35) : 12407-12Tamaru S, et al. Biochem Biophys Res Commun 2005 Jul 01; 332 (2) : 347-51Barkalow KL, et al. J Cell Biol 2003 May 12; 161 (3) : 557-70