14 3 3 zeta (phospho Ser58) Antibody

Product Specifications

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

NCBI Gene ID

7534

Swiss Prot

P63104

Accession Number

NP_001129171.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

14-3-3 zeta (Phospho-Ser58) antibody was raised against a peptide sequence around phosphorylation site of serine 58 (R-S-S (p) -W-R) derived from Human 14-3-3 zeta.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

28 kDa

Specificity

This antibody detects endogenous level of 14-3-3 zeta only when phosphorylated at serine 58.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

YWHAZ

NCBI Official Name

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta

NCBI Organism

Homo sapiens

Background Reference 01

Gu YM, et al. (2006) FEBS Lett ; 580 (1) : 305-310Powell DW, et al. (2003) Mol Cell Biol; 23 (15) : 5376-5387Mackintosh C. (2004) Biochem J; 381 (Pt 2) : 329-342.