ASK1 (phospho Ser966) Antibody

Product Specifications

Background

Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.

NCBI Gene ID

4217

Swiss Prot

Q99683

Accession Number

NP_005914.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

ASK1 (Phospho-Ser966) antibody was raised against a peptide sequence around phosphorylation site of serine 966 (S-I-S (p) -L-P) derived from Human ASK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

155 kDa

Specificity

This antibody detects endogenous level of ASK1 only when phosphorylated at serine 966.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

MAP3K5

NCBI Official Name

Mitogen-activated protein kinase kinase kinase 5

NCBI Organism

Homo sapiens

Background Reference 01

Zhang W, et al. (2005) J Biol Chem. 280 (19) : 19036-19044. Fujii K, et al. (2004) Oncogene. 23 (29) : 5099-5104. Goldman EH, et al. (2004) J Biol Chem 2004 Mar 12; 279 (11) : 10442-10449. Zhang L, et al. (1999) Proc Natl Acad Sci U S A. 96 (15) : 8511-8515.