AMPKa1 (phospho Ser487) Antibody

Product Specifications

Background

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

NCBI Gene ID

5562

Swiss Prot

Q13131; P54646

Accession Number

NP_006242.5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

AMPKa1 (Phospho-Ser487) antibody was raised in was raised against a peptide sequence around phosphorylation site of serine 487 (S-G- SP-V-S) derived from Human AMPKα1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

63 kDa

Specificity

This antibody detects endogenous level of AMPKα1 only when phosphorylated at serine 487.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

PRKAA1

NCBI Official Name

Protein kinase, AMP-activated, alpha 1 catalytic subunit

NCBI Organism

Homo sapiens

Background Reference 01

Kim JE, et al. (2005) J Proteome Res. 4 (4) : 1339-1346. Woods A, et al. (2003) J Biol Chem. 278 (31) : 28434-28442.

Other Product Names

AMPK, AMPKa1, FLK1, VEGFR2, AAPK1, AMPK alpha-1 chain, AMPK-alpha1, HMG-CoA redustase kinase, PRKAA1

Tested Applications

WB, IHC, IF

Protein ID

94557301

Physical Properties

Liquid

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