PKCtheta (phospho Ser695) Antibody

Product Specifications

Background

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR) -mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-κ -B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

NCBI Gene ID

5588

Swiss Prot

Q04759

Accession Number

NP_006248.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PKCtheta (Phospho-Ser695) antibody was raised against a peptide sequence around phosphorylation site of serine 695 (N-F-S (p) -F-M) derived from Human PKCΘ .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

80 kDa

Specificity

This antibody detects endogenous level of PKCΘ only when phosphorylated at serine 695.

Applications Notes

Western Blot: 1:500, Immunohistochemistry: 1:50~1:100

Symbol

PRKCQ

NCBI Official Name

Protein kinase C, theta

NCBI Organism

Homo sapiens

Background Reference 01

Xu ZB, et al. (2004) J Biol Chem 279:50401-50409Thebault S, et al. (2004) Mol Immunol 40: 931-942

Other Product Names

PRKCT, nPKC-theta, MMAC1, TEP1, KPCT, PKC-theta, PKCQ, PRKCQ, PRKCT

Tested Applications

WB, IHC

Protein ID

5453976

Physical Properties

Liquid

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