HSP27 (phospho Ser15) Antibody

Product Specifications

Background

Involved in stress resistance and actin organization.

NCBI Gene ID

3315

Swiss Prot

P04792

Accession Number

NP_001531.1

Host

Rabbit

Reactivity

Human

Immunogen

HSP27 (Phospho-Ser15) antibody was raised against a peptide sequence around phosphorylation site of serine15 (G-P-S (p) -W-D) derived from Human HSP27.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

27 kDa

Specificity

This antibody detects endogenous level of HSP27only when phosphorylated at serine 15.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

HSPB1

NCBI Official Name

Heat shock 27kDa protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Tremolada L, et al. (2005) Proteomics. 5 (3) : 788-795. McGregor E, et al. (2004) Mol Cell Proteomics. 3 (2) : 115-124. Benn SC, et al. (2002) Neuron. 36 (1) : 45-56. MacDonald JA, et al. (2002) Mol Cell Proteomics. 1 (4) : 314-322.

Other Product Names

CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, ASPL, RCC17, TUG, UBXD9, UBXN9, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, HSP25