MEK1 (phospho Ser221) Antibody

Product Specifications

Background

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

NCBI Gene ID

5604

Swiss Prot

Q02750

Accession Number

NP_002746.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

MEK1 (Phospho-Ser221) antibody was raised against a peptide sequence around phosphorylation site of serine 221 (A-N-S (p) -F-V) derived from Human MEK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

45 kDa

Specificity

This antibody detects endogenous level of MEK1 only when phosphorylated at serine 221.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

MAP2K1

NCBI Official Name

Mitogen-activated protein kinase kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Zebisch A, et al. (2006) Cancer Res; 66 (7) : 3401-8. Luciano BS, et al. (2006) J Biol Chem; 279 (50) : 52117-23. Wang X, et al. (2003) Oncogene; 22 (1) : 109-16. Gopalbhai K, et al. (2003) J Biol Chem; 278 (10) : 8118-25. Ling MT, et al. (2002) Oncogene; 21 (55) : 8498-505.