VASP (phospho Ser239) Antibody

Product Specifications

Background

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

NCBI Gene ID

7408

Swiss Prot

P50552

Accession Number

NP_003361.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

VASP (Phospho-Ser239) antibody was raised against a peptide sequence around phosphorylation site of serine 238 (K-V-S (p) -K-Q) derived from Human VASP.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

50 kDa

Specificity

This antibody detects endogenous level of VASP only when phosphorylated at serine 239.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

VASP

NCBI Official Name

Vasodilator-stimulated phosphoprotein

NCBI Organism

Homo sapiens

Background Reference 01

Wang HG, et al. (2005) euron; 45 (3) : 389-403. Melichar VO, et al. (2004) Proc Natl Acad Sci U S A; 101 (47) : 16671-6. Jensen BO, et al. (2004) Blood; 104 (9) : 2775-82. Zhuang S, et al. (2004) J Biol Chem; 279 (11) : 10397-407. Smolenski A, et al. (1998) J Biol Chem; 273 (32) : 20029-35. Butt E, et al. (1994) J Biol Chem; 269 (20) : 14509-17.