PAK1 (phospho Thr212) Antibody

Product Specifications

Background

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

NCBI Gene ID

5058

Swiss Prot

Q13153

Accession Number

NP_001122092.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PAK1 (Phospho-Thr212) antibody was raised against a peptide sequence around phosphorylation site of threonine 212 (P-V-T (p) -P-T) derived from Human PAK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

68 kDa

Specificity

This antibody detects endogenous level of PAK1 only when phosphorylated at threonine 212.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

PAK1

NCBI Official Name

P21 protein (Cdc42/Rac) -activated kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Alexander K, et al. (2004) Mol Cell Biol; 24: 2808-2819Thiel DA, et al. (2002) Curr Biol; 12:1227-1232Rashid T, et al. (2001) J. Biol. Chem; 276: 49043 - 49052.