What is the catalog number for IkB a (phospho Ser32 / Ser36) Antibody?

The catalog number for IkB a (phospho Ser32 / Ser36) Antibody is 79-175.

What sizes are available for IkB a (phospho Ser32 / Ser36) Antibody?

IkB a (phospho Ser32 / Ser36) Antibody is available in the following size(s): 0.1 mg.

Is IkB a (phospho Ser32 / Ser36) Antibody in stock?

IkB a (phospho Ser32 / Ser36) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

IkB a (phospho Ser32 / Ser36) Antibody

Product Specifications

Background

Inhibits the activity of dimeric NF-κ -B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

NCBI Gene ID

4792

Swiss Prot

P25963

Accession Number

NP_065390.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

IkB-a (Phospho-Ser32/Ser36) antibody was raised against a peptide sequence around phosphorylation site of serine 32/36 (H-D-S (p) -G-L-D-S (p) -M-K) derived from Human IkappaB-α .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

39 kDa

Specificity

This antibody detects endogenous level of IkappaB-α only when phosphorylated at serine 32/36.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

NFKBIA

NCBI Official Name

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

NCBI Organism

Homo sapiens

Background Reference 01

Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823. Honda, R. et al. (1997) FEBS Lett. 420, 25-27. Hirao, A. et al. (2000) Science 287, 1824-1827. Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157. Chehab, N. H. et al. (1999) Proc . Natl . Acad. Sci. USA 96, 13777-13782.