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MKK3 (phospho Ser189) Antibody

Product Specifications

Background

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.

NCBI Gene ID

5606

Swiss Prot

P46734

Accession Number

NP_002747.2

Host

Rabbit

Reactivity

Human, Rat

Immunogen

MKK3 (Phospho-Ser189) antibody was raised against a peptide sequence around phosphorylation site of serine 189 (V-D-S (p) -V-A) derived from Human MKK3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

40 kDa

Specificity

This antibody detects endogenous level of MKK3 only when phosphorylated at serine189.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

MAP2K3

NCBI Official Name

Mitogen-activated protein kinase kinase 3

NCBI Organism

Homo sapiens

Background Reference 01

Wang W, et al. (2002) Mol Cell Biol ; 22 (10) : 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16 (3) : 1247-55.

Other Product Names

MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2, MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MEK3

Tested Applications

WB, IHC, IF

Protein ID

21618347

Physical Properties

Liquid

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