Cofilin (phospho Ser3) Antibody
Product Specifications
Background
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
NCBI Gene ID
1072
Swiss Prot
P23528
Accession Number
NP_005498.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Cofilin (Phospho-Ser3) antibody was raised against a peptide sequence around phosphorylation site of serine 3 (M-A-S (p) -G-V) derived from Human cofilin.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=79-170
Calculated Molecular Weight
19 kDa
Specificity
This antibody detects endogenous level of cofilin only when phosphorylated at serine 3.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Symbol
CFL1
NCBI Official Name
Cofilin 1 (non-muscle)
NCBI Organism
Homo sapiens
Background Reference 01
Kobayashi M, et al. (2006) EMBO J 25 (4) : 713-26. Wang Y, et al. (2005) Biol Chem 280 (13) : 12683-9. Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.
Other Product Names
CFL, HEL-S-15, HER2, MLN19, NEU, NGL, CFL, CFL1
Tested Applications
WB, IHC, IF
Protein ID
5031635
Physical Properties
Liquid
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