Cofilin (phospho Ser3) Antibody

Product Specifications

Background

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

NCBI Gene ID

1072

Swiss Prot

P23528

Accession Number

NP_005498.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Cofilin (Phospho-Ser3) antibody was raised against a peptide sequence around phosphorylation site of serine 3 (M-A-S (p) -G-V) derived from Human cofilin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

19 kDa

Specificity

This antibody detects endogenous level of cofilin only when phosphorylated at serine 3.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

CFL1

NCBI Official Name

Cofilin 1 (non-muscle)

NCBI Organism

Homo sapiens

Background Reference 01

Kobayashi M, et al. (2006) EMBO J 25 (4) : 713-26. Wang Y, et al. (2005) Biol Chem 280 (13) : 12683-9. Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.