Cdc25A (phospho Ser76) Antibody
Product Specifications
Background
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
NCBI Gene ID
993
Swiss Prot
P30304
Accession Number
NP_001780.2
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Cdc25A (Phospho-Ser76) antibody was raised against a peptide sequence around phosphorylation site of serine 76 (M-G-S (p) -S-E) derived from Human cdc25A.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific, Cell Cycle
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
70 kDa
Specificity
This antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Symbol
CDC25A
NCBI Official Name
Cell division cycle 25A
NCBI Organism
Homo sapiens
Background Reference 01
Jin J, et al. (2003) Genes 17 (24) : 3062-74. Shimuta K, et al. (2002) EMBO J 21 (14) : 3694-703.
Other Product Names
CDC25A2, MEK1, PRKMK1, CDC25M3, MPIP1
Tested Applications
WB, IHC
Protein ID
42490758
Physical Properties
Liquid
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