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Cdc25A (phospho Ser76) Antibody

Product Specifications

Background

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

NCBI Gene ID

993

Swiss Prot

P30304

Accession Number

NP_001780.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Cdc25A (Phospho-Ser76) antibody was raised against a peptide sequence around phosphorylation site of serine 76 (M-G-S (p) -S-E) derived from Human cdc25A.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=79-169

Calculated Molecular Weight

70 kDa

Specificity

This antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CDC25A

NCBI Official Name

Cell division cycle 25A

NCBI Organism

Homo sapiens

Background Reference 01

Jin J, et al. (2003) Genes 17 (24) : 3062-74. Shimuta K, et al. (2002) EMBO J 21 (14) : 3694-703.

Other Product Names

CDC25A2, MEK1, PRKMK1, CDC25M3, MPIP1

Tested Applications

WB, IHC

Protein ID

42490758

Physical Properties

Liquid

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