AFX (phospho Ser197) Antibody

Product Specifications

Background

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.

NCBI Gene ID

4303

Swiss Prot

P98177

Accession Number

NP_001164402.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

AFX (Phospho-Ser197) antibody was raised against a peptide sequence around phosphorylation site of serine 197 (A-A-S (p) -M-D) derived from Human AFX.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

65 kDa

Specificity

This antibody detects endogenous level of AFX only when phosphorylated at serine 197.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

FOXO4

NCBI Official Name

Forkhead box O4

NCBI Organism

Homo sapiens

Background Reference 01

Di Maira G, et al. (2005) Cell Death Differ; 12 (6) : 668-77. Essers MA, et al. EMBO J 2004 Nov. 11. Brownawell AM, (2001) Mol Cell Biol; 21 (10) : 3534-46. Kops GJ, et al. (1999) Nature; 398 (6728) : 630-4.