FKHR (phospho Ser319) Antibody

Product Specifications

Background

FKHR belongs to the forkhead family of transcription factors, which are characterized by a distinct forkhead domain. It may play a role in myogenic growth and differentiation. The mammalian DAF-16-like transcription factors, FKHR, FKHRL1, and AFX, function as key regulators of insulin signaling, cell cycle progression, and apoptosis downstream of phosphoinositide 3-kinase. Gene activation through binding to insulin response sequences has been essential for mediating these functions. D-type Cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation. FKHR gene is mapped to chromosome 13q14

NCBI Gene ID

2308

Swiss Prot

Q12778

Accession Number

NP_002006.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

FKHR (Phospho-Ser319) antibody was raised against a peptide sequence around phosphorylation site of serine 319 (T-S-S (p) -N-A) derived from Human FKHR/FOXO1A.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

78 - 82 kDa

Specificity

This antibody detects endogenous level of FKHR only when phosphorylated at serine 319.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

FOXO1

NCBI Official Name

Forkhead box O1

NCBI Organism

Homo sapiens

Background Reference 01

Rena G, et al. (2002) EMBO J 21 (9) : 2263-2271. Woods YL, et al. (2001) Biochem J355 (Pt 3) : 597-607. Rena G, et al. (2001) Biochem J 354 (Pt 3) : 605-612.