CDC2 (phospho Thr161) Antibody

Product Specifications

Background

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

NCBI Gene ID

983

Swiss Prot

P06493

Accession Number

NP_001163877.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

CDC2 (Phospho-Thr161) antibody was raised against a peptide sequence around phosphorylation site of threonine161 (T-Y-T (p) -H-E) derived from Human CDC2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

34 kDa

Specificity

This antibody detects endogenous level of CDC2 only when phosphorylated at threonine 161.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CDK1

NCBI Official Name

Cyclin-dependent kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Ukomadu C, et al. (2003) J Biol Chem; 278 (7) : 4840-6. Morris MC, et al. (2002) J Biol Chem; 277 (26) : 23847-53. Brown NR, et al. (1999) J Biol Chem; 274 (13) : 8746-56. Liu Y, et al. (2004) J Biol Chem; 279 (6) : 4507-14.