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CDK2 (phospho Thr160) Antibody

Product Specifications

Background

Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.

NCBI Gene ID

1017

Swiss Prot

P24941

Accession Number

NP_001789.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

CDK2 (Phospho-Thr160) antibody was raised against a peptide sequence around phosphorylation site of threonine 160 (T-Y-T (p) -H-E) derived from Human CDK2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

34 kDa

Specificity

This antibody detects endogenous level of CDK2 only when phosphorylated at threonine 160.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CDK2

NCBI Official Name

Cyclin-dependent kinase 2

NCBI Organism

Homo sapiens

Background Reference 01

Ukomadu C, et al. (2003) J Biol Chem; 278 (7) : 4840-6. Morris MC, et al. (2002) J Biol Chem; 277 (26) : 23847-53. Brown NR, et al. (1999) J Biol Chem; 274 (13) : 8746-56. Liu Y, et al. (2004) J Biol Chem; 279 (6) : 4507-14.

Other Product Names

CDKN2, p33 (CDK2), JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1, kinase Cdk2, p33 protein kinase

Tested Applications

WB, IHC

Protein ID

16936528

Physical Properties

Liquid

Frequently Asked Questions

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