Rb (phospho Ser780) Antibody

Product Specifications

Background

Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

NCBI Gene ID

5925

Swiss Prot

P06400

Accession Number

NP_000312.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Rb (Phospho-Ser780) antibody was raised against a peptide sequence around phosphorylation site of serine 780 (T-L-S (p) -P-I) derived from Human Rb.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

110 kDa

Specificity

This antibody detects endogenous level of Rb only when phosphorylated at serine 780.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

RB1

NCBI Official Name

Retinoblastoma 1

NCBI Organism

Homo sapiens

Background Reference 01

Roesch A, et al. (2005) Mod Pathol. 18 (4) : 565-572. Huang H, et al. (2004) Oncogene. 23 (12) : 2161-2176. Kitagawa M, et al. (1996) EMBO J. 15 (24) : 7060-7069.