IKK a (phospho Thr23) Antibody

Product Specifications

Background

Acts as part of the IKK complex in the conventional pathway of NF-κ -B activation and phosphorylates inhibitors of NF-κ -B thus leading to the dissociation of the inhibitor/NF-κ -B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-κ -B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-κ -B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-κ -B-regulated promoters during inflammatory responses triggered by cytokines.

NCBI Gene ID

1147

Swiss Prot

O15111

Accession Number

NP_001269.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

IKK a (Phospho-Thr23) antibody was raised against a peptide sequence around phosphorylation site of threonine 23 (L-G-T (p) -G-G) derived from Human IKK α .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

85 kDa

Specificity

This antibody detects endogenous level of IKKα only when phosphorylated at threonine 23.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CHUK

NCBI Official Name

Conserved helix-loop-helix ubiquitous kinase

NCBI Organism

Homo sapiens

Background Reference 01

Yuan ZQ, et al. (2002) J Biol Chem; 277 (33) : 29973-82. Ozes ON, et al. (1999) Nature; 401 (6748) : 82-5.

Other Product Names

IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, CTNNB, OK/SW-cl.35, PRO2286, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK1

Tested Applications

WB, IHC

Protein ID

62241001

Physical Properties

Liquid

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