LIMK1 (phospho Thr508) Antibody

Product Specifications

Background

Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.

NCBI Gene ID

3984

Swiss Prot

P53667

Accession Number

NP_002305.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

LIMK1 (Phospho-Thr508) antibody was raised against a peptide sequence around phosphorylation site of threonine 508 (R-Y-T (p) -V-V) derived from Human LIMK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

72 kDa

Specificity

This antibody detects endogenous level of LIMK1 only when phosphorylated at threonine 508.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

LIMK1

NCBI Official Name

LIM domain kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Soosairajah J, et al. (2005) EMBO J. Ohashi K, et al. (2000) J Biol Chem; 275 (5) : 3577-82. Edwards DC, et al. (1999) Nat Cell Biol; 1 (5) : 253-9.

Other Product Names

LIMK, LIMK-1, C1orf215, LAP18, OP18, kinase LIMK1

Tested Applications

WB, IHC, IF

Protein ID

4505001

Physical Properties

Liquid

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