Akt2 (phospho Ser474) Antibody

Product Specifications

Background

General protein kinase capable of phosphorylating several known proteins.

NCBI Gene ID

208

Swiss Prot

P31751

Accession Number

NP_001617.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Akt2 (Phospho-Ser474) antibody was raised against a peptide sequence around phosphorylation site of serine 474 (Q-F-S (p) -Y-S) derived from Human Akt2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

60 kDa

Specificity

This antibody detects endogenous level of Akt2 only when phosphorylated at serine 474.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

AKT2

NCBI Official Name

V-akt murine thymoma viral oncogene homolog 2

NCBI Organism

Homo sapiens

Background Reference 01

Sun M, et al. (2001) Cancer Res; 61 (16) : 5985-91. Yuan ZQ, et al. (2000) Oncogene; 19 (19) : 2324-30. Meier R, et al. (1997) J Biol Chem; 272 (48) : 30491-7.