Cdc25C (phospho Ser216) Antibody
Product Specifications
Background
Cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
NCBI Gene ID
995
Swiss Prot
P30307
Accession Number
NP_001781.2
Host
Rabbit
Reactivity
Human
Immunogen
Cdc25C (Phospho-Ser216) antibody was raised against a peptide sequence around phosphorylation site of serine 216 (S-P-S (p) -M-P) derived from Human cdc25C.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific, Cell Cycle
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
60 kDa
Specificity
This antibody detects endogenous level of cdc25C only when phosphorylated at serine 216.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Symbol
CDC25C
NCBI Official Name
Cell division cycle 25C
NCBI Organism
Homo sapiens
Background Reference 01
Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3 (4) : 341-348. Ferguson AM. et al. (2005) Mol Cell Biol. 25 (7) : 2853-2860. Donzelli M. et al. (2003) EMBO Rep. 4 (7) : 671-677. Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99 (4) : 1990-1995.
Other Product Names
CDC25, PPP1R60, MACS, PRKCSL, CDC25M1, MPIP3
Tested Applications
WB, IHC, IF
Protein ID
125625350
Physical Properties
Liquid
Frequently Asked Questions
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