Cdc25C (phospho Ser216) Antibody

Product Specifications

Background

Cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

NCBI Gene ID

995

Swiss Prot

P30307

Accession Number

NP_001781.2

Host

Rabbit

Reactivity

Human

Immunogen

Cdc25C (Phospho-Ser216) antibody was raised against a peptide sequence around phosphorylation site of serine 216 (S-P-S (p) -M-P) derived from Human cdc25C.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

60 kDa

Specificity

This antibody detects endogenous level of cdc25C only when phosphorylated at serine 216.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

CDC25C

NCBI Official Name

Cell division cycle 25C

NCBI Organism

Homo sapiens

Background Reference 01

Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3 (4) : 341-348. Ferguson AM. et al. (2005) Mol Cell Biol. 25 (7) : 2853-2860. Donzelli M. et al. (2003) EMBO Rep. 4 (7) : 671-677. Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99 (4) : 1990-1995.