Tau (phospho Thr231) Antibody

Product Specifications

Background

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

NCBI Gene ID

4137

Swiss Prot

P10636

Accession Number

NP_001116538.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Tau (Phospho-Thr231) antibody was raised against a peptide sequence around phosphorylation site of threonine 231 (V-R-T (p) -P-P) derived from Human Tau.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

48, 62, 78 kDa

Specificity

This antibody detects endogenous level of Tau only when phosphorylated at threonine 231.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

MAPT

NCBI Official Name

Microtubule-associated protein tau

NCBI Organism

Homo sapiens

Background Reference 01

Yen S. H. et. al. (1997) Neurochem. 69:1709-1719. Beausoleil S. A. et. al. (2004) Proc. Natl. Acad. Sci. U. S. A. 101:12130-12135. Wintjens R. et. al. (2001) Biol. Chem. 276:25150-25156. Babu J. R. et. al. (2005) Neurochem. 94:192-203. Goedert M., Spillantini M. G. (2000) Biochim. Biophys. Acta 1502:110-121.