P90RSK (phospho Thr348) Antibody

Product Specifications

Background

Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.

NCBI Gene ID

6195

Swiss Prot

Q15418

Accession Number

NP_001006666.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

P90RSK (Phospho-Thr348) antibody was raised against a peptide sequence around phosphorylation site of threonine 348 (S-R-T (p) -P-R) derived from Human p90RSK.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

90 kDa

Specificity

This antibody detects endogenous level of p90RSK only when phosphorylated at threonine 348.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

RPS6KA1

NCBI Official Name

Ribosomal protein S6 kinase, 90kDa, polypeptide 1

NCBI Organism

Homo sapiens

Background Reference 01

Silverman E, et al. Mol Cell Biol. 2004 Dec; 24 (24) : 10573-10583. Andrew D, et al. Biochem J. 2006 February 1; 393 (Pt 3) : 715–724. Itoh S, et al. J Biol Chem 2005 Jun 24; 280 (25) : 24135-24142