P53 (phospho Ser15) Antibody

Product Specifications

Background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

NCBI Gene ID

7157

Swiss Prot

P04637

Accession Number

NP_000537.3

Host

Rabbit

Reactivity

Human

Immunogen

P53 (Phospho-Ser15) antibody was raised against a peptide sequence around phosphorylation site of serine 15 (P-L-S (p) -Q-E) derived from Human p53.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

53 kDa

Specificity

This antibody detects endogenous level of p53 only whenphosphorylated at serine15.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

TP53

NCBI Official Name

Tumor protein p53

NCBI Organism

Homo sapiens

Background Reference 01

Lin T, et al. (2005) Nat Cell Biol; 7 (2) : 165-71. Vega FM, et al. (2004) Mol Cell Biol; 24 (23) : 10366-80. Li J, et al. (2004) J Biol Chem; 279 (40) : 41275-9. Wang J, et al. (2004) J Biol Chem; 279 (38) : 39584-92.