Paxillin (phospho Tyr118) Antibody

Product Specifications

Background

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) .

NCBI Gene ID

5829

Swiss Prot

P49023

Accession Number

NP_001074324.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Paxillin (Phospho-Tyr118) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 118 (H-V-Y (p) -S-F) derived from Human Paxillin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

68 kDa

Specificity

This antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 118.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

PXN

NCBI Official Name

Paxillin

NCBI Organism

Homo sapiens

Background Reference 01

Tao WA, et al. (2005) Nat Methods. 2 (8) : 591-598. Zhang Y, et al. (2005) Mol Cell Proteomics. 4 (9) : 1240-1250. Sanders MA, et al. (2005) J Biol Chem. 280 (25) : 23516-23522. Rush J, et al. (2004) Nat Biotechnol. 23 (1) : 94-101.