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HER2 (phospho Tyr1248) Antibody

Product Specifications

Background

Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.

NCBI Gene ID

2064

Swiss Prot

P04626

Accession Number

NP_001005862.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

HER2 (Phospho-Tyr1248) antibody was raised against a peptide sequence around phosphorylation site of tyrosine1248 (P-E-Y (p) -L-G) derived from Human HER2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

185 kDa

Specificity

This antibody detects endogenous level of HER2 only when phosphorylated at tyrosine1248.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

ERBB2

NCBI Official Name

V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2

NCBI Organism

Homo sapiens

Background Reference 01

Yuan, Lu Qian, Ming Shi, et al. (2008) HER2-dependent MMP-7 expression is mediated by activated STAT3 Guogang Cellular Signalling, 20:1284–1291.

Other Product Names

NEU, NGL, HER2, TKR1, CD340, HER-2, MLN19, HER-2/neu, AMPK1, C-erbB-2, ErbB2

Tested Applications

WB, IHC, IF

Protein ID

54792098

Physical Properties

Liquid

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