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Estrogen Receptor a (phospho Ser118) Antibody

Product Specifications

Background

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

NCBI Gene ID

2099

Swiss Prot

P03372

Accession Number

NP_000116.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Estrogen Receptor-a (Phospho-Ser118) antibody was raised against a peptide sequence around phosphorylation site of serine 118 (Q-L-S (p) -P-F) derived from Human Estrogen Receptor-α .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

66 kDa

Specificity

This antibody detects endogenous level of Estrogen Receptor-α only when phosphorylated at serine 118.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

ESR1

NCBI Official Name

Estrogen receptor 1

NCBI Organism

Homo sapiens

Background Reference 01

Ramsauer VP, et al. (2003) J Biol Chem; 278 (32) : 30142-7. Jepson S, et al. (2002) Oncogene; 21 (49) : 7524-32. Ren Z, et al. (2002) J Biol Chem; 277 (41) : 38486-93. Eppenberger-Castori S, et al. (2001) J Clin Oncol; 19 (3) : 645-56.

Other Product Names

ER, ESR, Era, ESRA, ESTRR, NR3A1, BBC6, BCL2L8, ER, ESR1, ESTR, ESTRA

Tested Applications

WB, IHC, IF

Protein ID

62821794

Physical Properties

Liquid

Frequently Asked Questions

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