BAD (phospho Ser112) Antibody

Product Specifications

Background

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

NCBI Gene ID

572

Swiss Prot

Q92934

Accession Number

NP_004313.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

BAD (Phospho-Ser112) antibody was raised against a peptide sequence around phosphorylation site of serine 112 (H-S-S (p) -Y-P) derived from Human BAD.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

23 kDa

Specificity

This antibody detects endogenous level of BAD only when phosphorylated at serine 112.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

BAD

NCBI Official Name

BCL2-associated agonist of cell death

NCBI Organism

Homo sapiens

Background Reference 01

Zhang B, et al. (2004) . Mol Cell Biol. 24 (14) : 6205-6214. Rice PL, et al. (2003) . Cancer Res. 63 (3) : 616-620. Wang XQ, et al. (2001) . J Biol Chem. 276 (48) : 44504-44511.