Ezrin (phospho Tyr353) Antibody

Product Specifications

Background

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

NCBI Gene ID

7430

Swiss Prot

P15311

Accession Number

NP_001104547.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Ezrin (Phospho-Tyr353) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y (p) -E-E) derived from Human EZRIN.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

81 kDa

Specificity

This antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

EZR

NCBI Official Name

Ezrin

NCBI Organism

Homo sapiens

Background Reference 01

Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25) : 9485-9490. Wang Q, et al. (2003) J Biol Chem 278 (48) : 47731-47743. Gautreau A, et al. (1999) Proc Natl Acad Sci U S A 96 (13) : 7300-7305. Crepaldi T, et al. (1997) J Cell Biol 138 (2) : 423-34.