PTEN (phospho Ser380 / Thr382 / Thr383) Antibody

Product Specifications

Background

Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3, 4, 5-trisphosphate, phosphatidylinositol 3, 4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1, 3, 4, 5-tetrakisphosphate with order of substrate preference in vitro PtdIns (3, 4, 5) P3 > PtdIns (3, 4) P2 > PtdIns3P > Ins (1, 3, 4, 5) P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.

NCBI Gene ID

5728

Swiss Prot

P60484

Accession Number

NP_000305.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PTEN (Phospho-Ser380/Thr382/Thr383) antibody was raised against a peptide sequence around phosphorylation site of threonine 380/382/383 (R-Y-S (p) -D-T (p) -T (p) -D-S) derived from Human PTEN.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

54 kDa

Specificity

This antibody detects endogenous level of PTEN only when phosphorylated at serine 380 and threonine 382/383.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

PTEN

NCBI Official Name

Phosphatase and tensin homolog

NCBI Organism

Homo sapiens

Background Reference 01

Al-Khouri AM, et al. (2005) J Biol Chem. 280 (42) : 35195-35202. Torres J, et al. (2001) J Biol Chem. 276 (2) : 993-998. Vazquez F, et al. (2000) Mol Cell Biol. 20 (14) : 5010-5018.

Other Product Names

BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, Mutated in multiple advanced cancers 1, Protein-tyrosine phosphatase PTEN