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Akt (phospho Ser473) Antibody

Product Specifications

Background

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) . Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

NCBI Gene ID

207

Swiss Prot

P31749

Accession Number

NP_001014431.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Akt (Phospho-Ser473) antibody was raised against a peptide sequence around phosphorylation site of serine 473 (Q-F-S (p) -Y-S) derived from Human Akt.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=79-130

Calculated Molecular Weight

60 kDa

Specificity

This antibody detects endogenous level of Akt only when phosphorylated at serine 473.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

AKT1

NCBI Official Name

V-akt murine thymoma viral oncogene homolog 1

NCBI Organism

Homo sapiens

Background Reference 01

Baudhuin LM, et al. (2004) FASEB J Feb; 18 (2) : 341-3. Min YH, et al. (2004) Cancer Res; 64 (15) : 5225-31. Feng J, et al. (2004) J Biol Chem; 279 (34) : 35510-7.

Other Product Names

AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, NFKB3, C-AKT, PKB-alpha, RAC-PK-alpha

Tested Applications

WB, IHC

Protein ID

62241013

Physical Properties

Liquid

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