CREB (phospho Ser133) Antibody

Product Specifications

Background

CREB1 is a transcription factor that is a member of the bZIP family of DNA binding proteins. It binds as a homodimer to the CRE (cAMP-Responsive Element), an octameric palindrome containing a conserved core sequence, 5-prime-TGACG-3-prime. It consists of two transcript variants encoding respective isoforms produced by alternate splicing. It is mapped to 2q32.3-q34 . It is phosphorylated by several protein kinases and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB1 is crucial for the consolidation of long-term conditioned fear memories, but not for encoding, storage, or retrieval of these memories. It is required for the stability of reactivated or retrieved conditioned fear memories .

NCBI Gene ID

1385

Swiss Prot

P16220

Accession Number

NP_004370.1

Host

Rabbit

Reactivity

Human

Immunogen

CREB (Phospho-Ser133) antibody was raised against a peptide sequence around phosphorylation site of serine 133 (R-P-S (p) -Y-R) derived from Human CREB.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

43 kDa

Specificity

This antibody detects endogenous level of CREB only when phosphorylated at serine 133.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CREB1

NCBI Official Name

CAMP responsive element binding protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Xing J, et al. (1998) Mol Cell Biol 18 (4) : 1946-55. Tan Y, et al. (1996) EMBO J; 15 (17) : 4629-42. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Mayo LD, et al. (2001) Biol Chem; 276 (27) : 25184-9.