STAT3 (phospho Tyr705) Antibody

Product Specifications

Background

Transcription factor that binds to the interleukin-6 (IL-6) -responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.

NCBI Gene ID

6774

Swiss Prot

P40763

Accession Number

NP_003141.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

STAT3 (Phospho-Tyr705) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 705 (A-P-Y (p) -L-K) derived from Human STAT3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

88 kDa

Specificity

This antibody detects endogenous level of STAT1 only when phosphorylated at serine 727.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

STAT3

NCBI Official Name

Signal transducer and activator of transcription 3 (acute-phase response factor)

NCBI Organism

Homo sapiens

Background Reference 01

Fan G, et al. (2003) J Biol Chem. 278 (52) : 52432-52436. Barry FA, et al. (2003) FEBS Lett. 553 (1-2) : 173-178. Welsh, et al. (1996) Trends Cell Biol. 6: 274-279. Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.