Elk1 (phospho Ser389) Antibody
Product Specifications
Background
Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene
NCBI Gene ID
2002
Swiss Prot
P19419
Accession Number
NP_001107595.1
Host
Rabbit
Reactivity
Human
Immunogen
Elk1 (Phospho-Ser389) antibody was raised against a peptide sequence around phosphorylation site of serine 389 (P-R-S (p) -P-A) derived from Human Elk-1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=79-119
Calculated Molecular Weight
62 kDa
Specificity
This antibody detects endogenous level of Elk1 only when phosphorylated at serine 389.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Symbol
ELK1
NCBI Official Name
ELK1, member of ETS oncogene family
NCBI Organism
Homo sapiens
Background Reference 01
Janknecht R, et al. (1993) EMBO J. 12 (13) : 5097-5104. Marais R, et al. (1993) Cell 73:381-393. Kortenjann M, et al. (1994) Mol Cell Biol. 14:4815-4824. Hill C S, et al. (1995) Cell. 80:199-211. Cavigelli M, et al. (1995) EMBO J. 14:5957-5964.
Other Product Names
SUI1, ELK1, ETS-domain protein Elk-1
Tested Applications
WB, IHC
Protein ID
166362732
Physical Properties
Liquid
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