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Myc (phospho Thr58) Antibody

Product Specifications

Background

Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.

NCBI Gene ID

4609

Swiss Prot

P01106

Accession Number

NP_002458.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Myc (Phospho-Thr58) antibody was raised against a peptide sequence around phosphorylation site of threonine 58 (L-P-T (p) -P-P) derived from Human Myc.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=79-117

Calculated Molecular Weight

60 kDa

Specificity

This antibody detects endogenous level of Myc only when phosphorylated at threonine 58.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

MYC

NCBI Official Name

V-myc avian myelocytomatosis viral oncogene homolog

NCBI Organism

Homo sapiens

Background Reference 01

Jin Z, et al. (2004) J Biol Chem. 279 (38) : 40209-40219. Welcker M, et al. (2004) Proc Natl Acad Sci U S A. 101 (24) : 9085-9090. Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702. Blackwood E M, et al. (1991) Science. 251:1211-1217. Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.

Other Product Names

MRTL, MYCC, c-Myc, bHLHe39, BBC6, BCL2L8, c-myc

Tested Applications

WB, IHC

Protein ID

71774083

Physical Properties

Liquid

Curated Selection

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