ATF2 (phospho Thr71 / 53) Antibody
Product Specifications
Background
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
NCBI Gene ID
1386
Swiss Prot
P15336
Accession Number
NP_001871.2
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
ATF2 (Phospho-Thr71 or 53) antibody was raised against a peptide sequence around phosphorylation site of threonine 71 or 53 (T-P-T (p) -P-T) derived from Human ATF2.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
65 - 75 kDa
Specificity
This antibody detects endogenous level of ATF-2 only when phosphorylated at threonine 71 or 53.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Symbol
ATF2
NCBI Official Name
Activating transcription factor 2
NCBI Organism
Homo sapiens
Background Reference 01
Sevilla A, et al. (2004) J Biol Chem. 279 (26) : 27458-27465. Waetzig G H, et al. (2002) J Immunol. 168 (10) : 5342-5351. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. Van Dam H, et al. (1995) EMBO J. 14 (8) : 1798-1811.
Other Product Names
HB16, CREB2, TREB7, CREB-2, CRE-BP1, ERBB, ERBB1, HER1, CREBP1
Tested Applications
WB, IHC
Protein ID
22538422
Physical Properties
Liquid
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