ATF2 (phospho Ser62 / 44) Antibody

Product Specifications

Background

Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

NCBI Gene ID

1386

Swiss Prot

P15336

Accession Number

NP_001871.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

ATF2 (Phospho-Ser62 or 44) antibody was raised against a peptide sequence around phosphorylation site of serine 62 or 44 (N-D-S (p) -V-I) derived from Human ATF2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

65 - 75 kDa

Specificity

This antibody detects endogenous level of ATF-2 only when phosphorylated at serine 62 or 44.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

ATF2

NCBI Official Name

Activating transcription factor 2

NCBI Organism

Homo sapiens

Background Reference 01

Sevilla A, et al. (2004) J Biol Chem. 279 (26) : 27458-27465. Sakurai A, et al. (1991) Biochem Biophys Res Commun. 181 (2) : 629-635. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. Van Dam H, et al. (1995) EMBO J. 14 (8) : 1798-1811.