GSK3a (phospho Ser21) Antibody

Product Specifications

Background

Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.

NCBI Gene ID

2931

Swiss Prot

P49840

Accession Number

NP_063937.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

GSK3a (Phospho-Ser21) antibody was raised against a peptide sequence around phosphorylation site of serine 21 (T-S-S (p) -F-A) derived from Human GSK3α .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

51 kDa

Specificity

This antibody detects endogenous level of GSK3α only when phosphorylated at serine 21and does not detect GSK-3β when phosphorylated at Ser9.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

GSK3A

NCBI Official Name

Glycogen synthase kinase 3 alpha

NCBI Organism

Homo sapiens

Background Reference 01

Barry FA, et al. (2003) FEBS Lett. 553 (1-2) : 173-178. Koivisto L, et al. (2003) J Cell Sci. 116 (Pt 18) : 3749-3760. Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279. Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.