Raf1 (phospho Ser259) Antibody
Product Specifications
Background
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
NCBI Gene ID
5894
Swiss Prot
P04049
Accession Number
NP_002871.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Raf1 (Phospho-Ser259) antibody was raised against a peptide sequence around phosphorylation site of serine 259 (S-T-S (p) -T-P) derived from Human Raf1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific, Apoptosis, Cancer
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=79-104
Calculated Molecular Weight
73 kDa
Specificity
This antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Symbol
RAF1
NCBI Official Name
V-raf-1 murine leukemia viral oncogene homolog 1
NCBI Organism
Homo sapiens
Background Reference 01
Dougherty M K, et al. (2005) Mol Cell. 17 (2) : 215-224. Hekman M, et al. (2005) FEBS Lett. 579 (2) : 464-468. Avruch J, et al. (1994) Trends Biochem. Sci. 19, 279-283. Chong H, et al. (2001) EMBO J. 20, 3716-3727. King A J, et al. (1998) Nature. 396:180-183.
Other Product Names
NS5, CRAF, Raf-1, c-Raf, BBC6, BCL2L8, C-RAF, C-Raf
Tested Applications
WB, IHC
Protein ID
4506401
Physical Properties
Liquid
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