What is the catalog number for Raf1 (phospho Ser259) Antibody?

The catalog number for Raf1 (phospho Ser259) Antibody is 79-104.

What sizes are available for Raf1 (phospho Ser259) Antibody?

Raf1 (phospho Ser259) Antibody is available in the following size(s): 0.1 mg.

Is Raf1 (phospho Ser259) Antibody in stock?

Raf1 (phospho Ser259) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Raf1 (phospho Ser259) Antibody

Product Specifications

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

NCBI Gene ID

5894

Swiss Prot

P04049

Accession Number

NP_002871.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Raf1 (Phospho-Ser259) antibody was raised against a peptide sequence around phosphorylation site of serine 259 (S-T-S (p) -T-P) derived from Human Raf1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

73 kDa

Specificity

This antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

RAF1

NCBI Official Name

V-raf-1 murine leukemia viral oncogene homolog 1

NCBI Organism

Homo sapiens

Background Reference 01

Dougherty M K, et al. (2005) Mol Cell. 17 (2) : 215-224. Hekman M, et al. (2005) FEBS Lett. 579 (2) : 464-468. Avruch J, et al. (1994) Trends Biochem. Sci. 19, 279-283. Chong H, et al. (2001) EMBO J. 20, 3716-3727. King A J, et al. (1998) Nature. 396:180-183.