Raf1 (phospho Ser259) Antibody

Product Specifications

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

NCBI Gene ID

5894

Swiss Prot

P04049

Accession Number

NP_002871.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Raf1 (Phospho-Ser259) antibody was raised against a peptide sequence around phosphorylation site of serine 259 (S-T-S (p) -T-P) derived from Human Raf1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

73 kDa

Specificity

This antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

RAF1

NCBI Official Name

V-raf-1 murine leukemia viral oncogene homolog 1

NCBI Organism

Homo sapiens

Background Reference 01

Dougherty M K, et al. (2005) Mol Cell. 17 (2) : 215-224. Hekman M, et al. (2005) FEBS Lett. 579 (2) : 464-468. Avruch J, et al. (1994) Trends Biochem. Sci. 19, 279-283. Chong H, et al. (2001) EMBO J. 20, 3716-3727. King A J, et al. (1998) Nature. 396:180-183.