PDK1 (phospho Ser241) Antibody

Product Specifications

Background

Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-ζ , RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.

NCBI Gene ID

5170

Swiss Prot

O15530

Accession Number

NP_002604.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PDK1 (Phospho-Ser241) antibody was raised against a peptide sequence around phosphorylation site of serine 241 (A-N-S (p) -F-V) derived from Human PDK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

63 kDa

Specificity

This antibody detects endogenous level of PDK1 only when phosphorylated at serine 241.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

PDPK1

NCBI Official Name

3-phosphoinositide dependent protein kinase-1

NCBI Organism

Homo sapiens

Background Reference 01

Scheid MP, et al. (2005) Mol Cell Biol; 25 (6) : 2347-63Chen H, et al. (2001) Biochemistry; 40 (39) : 11851-9Sato S, et al. (2002) J Biol Chem; 277 (42) : 39360-7Lim MA, et al. (2003) Proc Natl Acad Sci U S A; 100 (24) : 14006-11