C Jun (phospho Ser73) Antibody

Product Specifications

Background

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

NCBI Gene ID

3725

Swiss Prot

P05412

Accession Number

NP_002219.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

C-Jun (Phospho-Ser73) antibody was raised against a peptide sequence around phosphorylation site of serine 73 (L-A-S (p) -P-E) derived from Human c-Jun.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific , Inflammasomes

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

43 kDa

Specificity

This antibody detects endogenous level of c-Jun only when phosphorylated at serine 73.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

JUN

NCBI Official Name

Jun proto-oncogene

NCBI Organism

Homo sapiens

Background Reference 01

Sevilla A, et al. (2004) Oncogene. 23 (55) : 8950-8958. Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101 (33) : 12130-12135. Binetruy B, et al. (1991) Nature. 351:122-127. Smeal T, et al. (1991) Nature. 354: 494-496. Derijard B, et al. (1994) Cell. 76:1025-1037.