APAF1 Antibody
Product Specifications
Background
Apoptotic protease activating factor 1. Apaf-1 is a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC) . Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
NCBI Gene ID
317
Swiss Prot
O14727
Accession Number
NP_863658.1
Host
Chicken
Reactivity
Human, Mouse
Immunogen
307-388
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Apoptosis, Cancer
Purification
Antigen affinity-purified
Concentration
1 mg/mL
Buffer
Phosphate-Buffered Saline. No preservatives added.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
APAF1 antibody can be stored at 4˚ C for short term (weeks) . Long term storage should be at -20˚ C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=XW-7034
Calculated Molecular Weight
141.8 kDa (calculated)
Applications Notes
Apaf-1 antibody can be used for the detection of Apaf-1 by Western blot, may also work for IHC and ICC.
Symbol
APAF1
NCBI Official Name
Apoptotic peptidase activating factor 1
NCBI Organism
Homo sapiens
Background Reference 01
Cecconi, F., Alvarez-Bolado, G., Meyer, B. I., Roth, K. A. and Gruss, P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 94 (6), 727-737 (1998)
Other Product Names
KIAA0413, Apoptotic protease-activating factor 1APAF-1, CED4, APAF-1
Tested Applications
WB
Protein ID
32483361
Physical Properties
Liquid
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