NPT Antibody [4B4D1]
Product Specifications
Background
Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph (3') -II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.
NCBI Gene ID
11979140
Swiss Prot
Q79C30
Accession Number
Q79C30
Host
Mouse
Reactivity
Human
Immunogen
Ni-NTA purified truncated recombinant NPT expressed in E. Coli strain BL21 (DE3) .
Clonality
Monoclonal
Clone
4B4D1
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Signal Transduction
Purification
Antibody is purified by protein A affinity chromatography.
Concentration
Batch dependent
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
NPT monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=32-199
Fragment
IgG2a
Symbol
Npt
NCBI Official Name
Neomycin phosphotransferase
NCBI Organism
Plasmid pRL765
Background Reference 01
Landis WG et.al Human and ecological Risk Assessment 6 (5) : 875-899.
Background Reference 02
Stewart CN et.al BioTechniques 29 (4) : 832-843.
Background Reference 03
Thomson JA et.al Journal of Food Science 66 (2) : 188-193.
Tested Applications
ELISA, WB
Protein ID
81919445
Physical Properties
Liquid
Curated Selection
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