4E-BP1 Antibody [11G12C11]

Product Specifications

Background

4E-BP1 (eukaryotic translation Initiation Factor 4E Binding Protein 1), also called ELF4EBP1/BP-1/PHAS-I , which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa) . Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while (24 kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

NCBI Gene ID

1978

Swiss Prot

Q13541

Accession Number

Q13541

Host

Mouse

Reactivity

Human

Immunogen

Ni-NTA purified truncated recombinant 4EBP1 expressed in E. Coli strain BL21 (DE3) .

Clonality

Monoclonal

Clone

11G12C11

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

Antibody is purified by protein A affinity chromatography.

Concentration

Batch dependent

Buffer

Purified antibody in PBS containing 0.03% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

4E-BP1 monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.