HLA-DRB5 Antibody

Product Specifications

Background

HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

NCBI Gene ID

3127

Swiss Prot

Q30154

Accession Number

Q30154

Host

Rabbit

Reactivity

Human

Immunogen

This HLA-DRB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the Central region of human HLA-DRB5.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

30 kDa

Fragment

Rabbit Ig

Symbol

HLA-DRB5

NCBI Official Name

HLA class II histocompatibility antigen, DR beta 5 chain

NCBI Organism

Homo sapiens

Background Reference 01

Asano, K., et al. Nat. Genet. 41 (12) :1325-1329 (2009)

Background Reference 02

Barrett, J.C., et al. Nat. Genet. 41 (12) :1330-1334 (2009)

Background Reference 03

Montano-Loza, A.J., et al. Liver Int. 29 (10) :1543-1551 (2009)

Background Reference 04

Lincoln, M.R., et al. Proc. Natl. Acad. Sci. U.S.A. 106 (18) :7542-7547 (2009)

Other Product Names

HLA class II histocompatibility antigen, DR beta 5 chain, DR beta-5, DR2-beta-2, Dw2, MHC class II antigen DRB5, HLA-DRB5 {ECO:0000312|EMBL:CAI180791}