NEK9 Antibody
Product Specifications
Background
NEK9, a member of the NIMA subfamily of Ser/Thr protein kinases, is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. It phosphorylates different histones (serine and threonine residues on H3), myelin basic protein, beta-casein (serine residues), and BICD2. NEK9 is activated during mitosis by intramolecular autophosphorylation. Activity and autophosphorylation is activated by manganese >> magnesium ions, and is sensitive to increasing concentration of detergents. This protein is not cell-cycle regulated but activity is higher in G0-arrested cells. NEK is part of a homodimer that binds to Ran GTPase, and exhibits a greater affinity for Ran-GDP over Ran-GTP. Interaction is also noted with NEK6 and NEK7 family members. This cytoplasmic protein is most abundant in heart, liver, kidney and testis, and is also expressed in smooth muscle cells and fibroblasts. Expression varies mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.
NCBI Gene ID
91754
Swiss Prot
Q8TD19
Accession Number
Q8TD19
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
This NEK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 846-877 amino acids from the C-terminal region of human NEK9.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle, Signal Transduction
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=63-383
Calculated Molecular Weight
107 kDa
Fragment
Rabbit Ig
Symbol
NEK9
NCBI Official Name
Serine/threonine-protein kinase Nek9
NCBI Organism
Homo sapiens
Background Reference 01
Tan, B.C. et al. J. Biol. Chem. 279 (10), 9321-9330 (2004)
Background Reference 02
Belham, C. et al. J. Biol. Chem. 278 (37), 34897-34909 (2003)
Background Reference 03
Roig, J. et al. Genes Dev. 16 (13) :1640-1658 (2002) .
Background Reference 04
Holland, P.M. et al. J. Biol. Chem. 277 (18) :16229-16240 (2002) .
Other Product Names
Serine/threonine-protein kinase Nek9, Nercc1 kinase, Never in mitosis A-related kinase 9, NimA-related protein kinase 9, NimA-related kinase 8, Nek8, NEK9, KIAA1995, NEK8, NERCC
Tested Applications
WB, IHC-P
Protein ID
116242675
Physical Properties
Liquid
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